Industry Applications

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Industry Applications

Buffer Preparation

In pharmaceutical and biological product manufacturing, buffers are essential in many purification processes, such as chromatography, ultrafiltration, dialysis, and formulation. The filtration of buffers is crucial for ensuring production efficiency and the quality of the final product. To extend the service life of chromatography columns and downstream ultrafiltration systems, as well as to guarantee the endotoxin-free nature of the final product, sterile-grade filters are commonly used to filter buffers. The use of prefilters can further prolong the service life of the terminal sterile filters.

Purpose of Separation

● Prefiltration

To reduce particle content and biological burden before sterile filtration.

● Terminal Filtration

To remove bacteria and particles.

Application Requirements

● Buffers often contain sodium acetate, phosphate, and sodium phosphate. Generally, buffers with salt concentrations less than 1M do not require prefiltration.

● If buffers are not prepared using reagent-grade raw materials or contain high concentrations of salts, prefiltration is generally necessary. Proper configuration of prefilters and sterile filters can achieve high flow rates and stable high throughput.